Bushen Huoxue Recipe enhances the immune microenvironment at the maternal-fetal interface via the Hippo signaling pathway in mice with recurrent spontaneous abortion

According to network pharmacology, BSHXR may act on the Toll-like receptor signaling pathway and Th17 cell differentiation. BSHXR significantly decreased the embryo absorption rate of RSA mice. The mRNA and protein expression levels of FoxP3 were increased in the BSHXR group, whereas the expression levels of TLR2, TLR4, TLR7 and RORγt were decreased. YAP/TAZ nuclear translocation was inhibited in the BSHXR group, and it might also regulate the Treg/Th17 immune balance by activating the Hippo pathway. Taken together, the findings of this study indicate that BSHXR may reduce pregnancy loss by regulating the immune microenvironment at the maternal-fetal interface, which provides a theoretical basis for its clinical application and future investigations.