Analysis of Gene Regulatory Network and Transcription Factors in Different Tissues of the Stropharia rugosoannulata Fruiting Body

<i>Stropharia rugosoannulata</i> is a mushroom that is rich in nutrients and has a pleasant flavor. Its cultivation area is expanding rapidly due to its simplicity and diversity. However, the developmental mechanism of the fruiting body, which constitutes the edible portion of <i>S. rugosoannulata</i>, remains to be elucidated. To address this knowledge gap, we conducted a comprehensive study. Our approach entailed the observation of sections through the fruiting body of <i>S. rugosoannulata</i> and the sequencing of the transcriptomes of various fruiting body tissues. The results demonstrated significant variations in the structure of the pileipellis, pileus, gill, veil, stipe, and trama of <i>S. rugosoannulata</i>. The predominant metabolic pathways included the amino acid metabolism of the pileus, sugar metabolism of the stipe, tryptophan metabolism, and wax production of the pileipellis, the DNA pathway of the gill, amino sugar metabolism of the veil, and the nitrogen metabolism of the trama. The promoter cis-element analysis revealed the roles of light response, methyl jasmonate, oxygen, and temperature on the differentiation of the veil, trama, and pileipellis, respectively. In summary, the present findings offer a molecular mechanism for the development of the fruiting body and provide directions for the enhancement of cultivation techniques of <i>S. rugosoannulata</i>.