Tamlana flava sp. nov., isolated from mangrove sediment and genome-based taxonomic analysis of the genus Tamlana

A novel yellow-coloured bacterial strain MA10^T was isolated from mangrove sediment and subjected to polyphasic taxonomic identification. Strain MA10^T was Gram-negative, rod-shaped, catalase-positive and oxidase-positive. Carotenoid pigment was present, and flexirubin-type pigment was absent. The 16S rRNA gene of strain MA10^T had the highest sequence similarity with <i>Tamlana crocina</i> HST1-43^T of 94.5%. The genome size was 3.77 Mbp with a genomic G+C content of 36.3%. The phylogenetic analysis of the 16S rRNA gene sequence and whole-genome sequence showed that strain MA10^T belonged to the genus <i>Tamlana</i> of the family <i>Flavobacteriaceae</i> and tightly clustered with <i>T. crocina</i> HST1-43^T. The digital DNA-DNA hybridization value and average nucleotide identity value between strain MA10^T and <i>T. crocina</i> HST1-43^T were 20.4 and 76.7%, respectively. The major menaquinone was MK-6. The major fatty acids (>10 %) were iso-C_15:0 (41.6%) and iso-C_15:1 G (16.8%). The polar lipids consisted of phosphatidylethanolamine, two unidentified aminolipids and three unidentified lipids. Based on the present polyphasic taxonomic study, strain MA10^T was considered to represent a novel species of the genus <i>Tamlana</i>, for which the name <i>Tamlana flava</i> sp. nov. was proposed. The type strain was MA10^T (=MCCC 1K09289^T=KCTC 102321^T). Additionally, the phylogeny of the 16S rRNA gene and whole-genome sequences found that the hitherto described nine species of the genus <i>Tamlana</i> and <i>Algibacter onchidii</i> presented polyphyletic clades, which could be divided into five different genera. The average amino acid identity value of 80% was chosen as the boundary of the five genera. Thus, except for the genus <i>Tamlana</i>, four novel genera named <i>Allotamlana</i> gen. nov., <i>Cognatitamlana</i> gen. nov., <i>Neotamlana</i> gen. nov. and <i>Pseudotamlana</i> gen. nov. were proposed. This study provided valuable taxonomic analysis of the genus <i>Tamlana</i>.