Correlation-based nanometric localization using lattice SIM2 and dSTORM in PML nuclear bodies validated by calibration spheres

We evaluated the Lattice di-SIM 3D structural illumination method with a focus on Promyelocytic Leukemia Nuclear Bodies (PML NBs). Lattice SIM<sup>2</sup>'s performance was compared to its predecessor using biological samples and calibration beads. Optimized SIM<sup>2</sup> parameters revealed PML NBs with a distinct ring-like morphology. Furthermore, we compared Lattice-SIM<sup>2</sup> with dSTORM, finding a strong correlation between blinking events and the SIM<sup>2</sup> mask. For 2D nanoscopic correlation, we used the long-lived imaging buffer Eternity, while the modified variant, Eternity-SIM, with a higher refractive index, provided improved 3D correlation for SIM<sup>2</sup> imaging.

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Publication Details

Journal
Biochemical and Biophysical Research Communications
Published
2025-02-07
DOI
https://doi.org/10.1016/j.bbrc.2025.151450
Citations
1
Primary Topic
Advanced Electron Microscopy Techniques and Applications
Type
article
Field-Weighted Citation Impact
2.55

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article

Correlation-based nanometric localization using lattice SIM2 and dSTORM in PML nuclear bodies validated by calibration spheres

Karine Monier, Elodie Chatre, Corentin Rousset, Franceline Juillard et al.
1 citations
Biochemical and Biophysical Research Communications
Advanced Electron Microscopy Techniques and Applications
2.55
article

Correlation-based nanometric localization using lattice SIM2 and dSTORM in PML nuclear bodies validated by calibration spheres

Karine Monier, Elodie Chatre, Corentin Rousset, Franceline Juillard, Patrick Lomonte, Christophe Place, Arnaud Favier, Rémi Néplaz, Pelin Catal
article en
1 citations

Abstract

We evaluated the Lattice di-SIM 3D structural illumination method with a focus on Promyelocytic Leukemia Nuclear Bodies (PML NBs). Lattice SIM<sup>2</sup>'s performance was compared to its predecessor using biological samples and calibration beads. Optimized SIM<sup>2</sup> parameters revealed PML NBs with a distinct ring-like morphology. Furthermore, we compared Lattice-SIM<sup>2</sup> with dSTORM, finding a strong correlation between blinking events and the SIM<sup>2</sup> mask. For 2D nanoscopic correlation, we used the long-lived imaging buffer Eternity, while the modified variant, Eternity-SIM, with a higher refractive index, provided improved 3D correlation for SIM<sup>2</sup> imaging.

Biochemical and Biophysical Research CommunicationsVol. 752
Université Claude Bernard Lyon 1 (FR), École Normale Supérieure de Lyon (FR), Centre National de la Recherche Scientifique (FR), Inserm (FR), Centre de Recherche en Neurosciences de Lyon (FR), Laboratoire de Physique de l'ENS de Lyon (FR), Institut NeuroMyoGène (FR), Institut National des Sciences Appliquées de Lyon (FR)
Agence Nationale de la Recherche, Centre National de la Recherche Scientifique, AFM-Téléthon
Openalex Percentile: Top 12%
Advanced Electron Microscopy Techniques and Applications
2.55
DOIOpen Access PDFOpenAlex
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Correlation-based nanometric localization using lattice SIM2 and dSTORM in PML nuclear bodies validated by calibration spheres — Karine Monier, Elodie Chatre, et al. · Biochemical and Biophysical Research Communications (2025) | TGRS Research Map | TGRS