Improved limit of detection for zoonotic Plasmodium knowlesi and P. cynomolgi surveillance using reverse transcription for total nucleic acid preserved samples or dried blood spots
Our findings support the use of an 18S rRNA Plasmodium genus qPCR and species-specific nested PCR protocol with RT for highly-sensitive surveillance of zoonotic and human Plasmodium species infections.
Authors
- Nathaniel Christy
- Henry Surendra (ORCID: https://orcid.org/0000-0002-6029-0623)
- Angelica F. Tan (ORCID: https://orcid.org/0000-0001-7914-758X)
- Giri Shan Rajahram (ORCID: https://orcid.org/0000-0002-8123-476X)
- Ranti Permatasari
- Andrew G. Letizia
- Sylvia Daim (ORCID: https://orcid.org/0000-0003-0065-5712)
- Inke Nadia Diniyanti Lubis (ORCID: https://orcid.org/0000-0001-7075-9491)
- Irbah Rea Alvieda Nainggolan
- Timothy William (ORCID: https://orcid.org/0000-0002-2195-6736)
- Rintis Noviyanti (ORCID: https://orcid.org/0000-0003-1383-3274)
- Matthew J. Grigg (ORCID: https://orcid.org/0000-0001-9914-8352)
- Sitti Saimah binti Sakam
- Bridget E. Barber (ORCID: https://orcid.org/0000-0003-1066-7960)
- Leily Trianty (ORCID: https://orcid.org/0000-0002-4368-476X)
- Jacob A. F. Westaway (ORCID: https://orcid.org/0000-0002-5171-819X)
- Kim A. Piera (ORCID: https://orcid.org/0000-0002-7414-9604)
- Kamil A. Braima (ORCID: https://orcid.org/0000-0003-2529-2313)
- Christopher L. Peatey
- Mohd Arshil Moideen
- Ristya Amalia (ORCID: https://orcid.org/0009-0003-3013-8967)
- Pinkan Kariodimedjo (ORCID: https://orcid.org/0009-0003-3333-9596)
- Lee C (ORCID: https://orcid.org/0000-0002-4280-0829)
- Nicholas M. Anstey
- Colin J. Sutherland
Institutions
- Ministry of Health (MY)
- Monash University Malaysia (MY)
- Universiti of Malaysia Sabah (MY)
- QIMR Berghofer Medical Research Institute (AU)
- Universitas Sumatera Utara (ID)
- Eijkman Institute for Molecular Biology (ID)
- Australian Institute of Tropical Health and Medicine (AU)
- University of Indonesia (ID)
- Charles Darwin University (AU)
- Menzies School of Health Research (AU)
- London School of Hygiene & Tropical Medicine (GB)
- Queen Elizabeth Hospital (MY)
- Oxford University Clinical Research Unit Indonesia
- National Defence University of Malaysia (MY)
- James Cook University (AU)
Publication Details
- Journal
- PLoS neglected tropical diseases
- Published
- 2025-02-06
- DOI
- https://doi.org/10.1371/journal.pntd.0012129
- Citations
- 6
- Primary Topic
- Malaria Research and Control
- Type
- article
- Field-Weighted Citation Impact
- 11.27
Funders
- U.S. Department of Defense
- University of Otago
- Australian Government
- Department of Foreign Affairs and Trade, Australian Government
- Kementerian Kesihatan Malaysia
- National Institutes of Health
- Defense Health Agency
- Medical Research Council
- National Health and Medical Research Council
- Australian Centre for International Agricultural Research
- National Institute of Allergy and Infectious Diseases