A single-nucleus RNA sequencing atlas of the postnatal retina of the shark Scyliorhinus canicula
The retina, whose basic cellular structure is highly conserved across vertebrates, constitutes an accessible system for studying the central nervous system. In recent years, single-cell RNA sequencing studies have uncovered cellular diversity in the retina of a variety of species, providing new insights on retinal evolution and development. However, similar data in cartilaginous fishes, the sister group to all other extant jawed vertebrates, are still lacking. Here, we present a single-nucleus RNA sequencing atlas of the postnatal retina of the catshark Scyliorhinus canicula, consisting of the expression profiles for 17,438 individual cells from three female, juvenile catshark specimens. Unsupervised clustering revealed 22 distinct cell types comprising all major retinal cell classes, as well as retinal progenitor cells (whose presence reflects the persistence of proliferative activity in postnatal stages in sharks) and oligodendrocytes. Thus, our dataset serves as a foundation for further studies on the development and function of the catshark retina. Moreover, integration of our atlas with data from other species will allow for a better understanding of vertebrate retinal evolution.
Authors
- Eva Candal (ORCID: https://orcid.org/0000-0001-6136-7605)
- Céline Schneider (ORCID: https://orcid.org/0000-0002-4505-4825)
- Ismael Hernández‐Núñez (ORCID: https://orcid.org/0000-0002-0313-8465)
- Sarah J. Salisbury (ORCID: https://orcid.org/0000-0001-7637-7742)
- Francesco Lamanna (ORCID: https://orcid.org/0000-0002-0447-759X)
- Sylvie Mazan (ORCID: https://orcid.org/0000-0003-4657-8097)
- Paula R. Villamayor (ORCID: https://orcid.org/0000-0002-7361-7795)
- Xuefei Yuan (ORCID: https://orcid.org/0000-0001-5193-9964)
- Diego Robledo (ORCID: https://orcid.org/0000-0002-9616-5912)
- Fátima Adrio (ORCID: https://orcid.org/0000-0002-3196-7682)
- Antón Barreiro‐Iglesias (ORCID: https://orcid.org/0000-0002-7507-080X)
- Francisca Hervas-Sotomayor (ORCID: https://orcid.org/0009-0007-0965-2493)
- Henrik Kaessmann (ORCID: https://orcid.org/0000-0001-7563-839X)
- Nicolás Vidal-Vázquez (ORCID: https://orcid.org/0000-0001-8899-9522)
- Pablo Carballo-Pacoret (ORCID: https://orcid.org/0009-0005-4095-7637)
- Julia Schmidt (ORCID: https://orcid.org/0000-0002-4617-3463)
Institutions
- Centre National de la Recherche Scientifique (FR)
- Roslin Institute (GB)
- Universidade de Santiago de Compostela (ES)
- Heidelberg University (DE)
- Sorbonne Université (FR)
- Institut National de Recherche pour l'Agriculture, l'Alimentation et l'Environnement (FR)
- Biologie Intégrative des Organismes Marins (FR)
- Laboratoire de Physiologie et Génomique des Poissons (FR)
- DKFZ-ZMBH Alliance (DE)
- University of Edinburgh (GB)
Publication Details
- Journal
- Scientific Data
- Published
- 2025-02-07
- DOI
- https://doi.org/10.1038/s41597-025-04547-2
- Citations
- 4
- Primary Topic
- Single-cell and spatial transcriptomics
- Type
- article
- Field-Weighted Citation Impact
- 3.22
Funders
- Ministerio de Ciencia, Innovación y Universidades
- European Commission
- Xunta de Galicia
- Natural Sciences and Engineering Research Council of Canada
- Biotechnology and Biological Sciences Research Council
- European Regional Development Fund
- Agencia Estatal de Investigación
- Spanish National Plan for Scientific and Technical Research and Innovation