Targeted detection of endogenous LINE-1 proteins and ORF2p interactions
This is the first assay to demonstrate sensitive and robust quantitation of endogenous ORF2p. The ability to assay ORF2p directly and quantitatively will improve our understanding of the developmental and diseased cell states where L1 expression and its activity naturally occur. The ability to simultaneously assay endogenous L1 ORF1p and ORF2p is an important step forward for L1 analytical biochemistry. Endogenous ORF2p interactomes can now be presented with confidence that ORF2p is among the enriched proteins.
Authors
- Martin S. Taylor (ORCID: https://orcid.org/0000-0001-7656-330X)
- Samira Hozeifi
- John LaCava (ORCID: https://orcid.org/0000-0002-6307-7713)
- Vikram Deshpande (ORCID: https://orcid.org/0000-0003-4230-9308)
- Alienke van Pijkeren (ORCID: https://orcid.org/0000-0001-6830-0606)
- Mehrnoosh Oghbaie (ORCID: https://orcid.org/0000-0002-8225-3436)
- Mathias I. Nielsen (ORCID: https://orcid.org/0000-0002-6823-5354)
- Omar G. Rosas Bringas
- Justina C. Wolters (ORCID: https://orcid.org/0000-0003-0066-3720)
- Brian T. Chait (ORCID: https://orcid.org/0000-0003-3524-557X)
- Luciano H. Di Stefano
- Lars ter Morsche (ORCID: https://orcid.org/0000-0003-2725-963X)
- Hua Jiang (ORCID: https://orcid.org/0000-0002-7561-3085)
- Marieke Smit
- Apostolos Mourtzinos
- Mats J. Nitert
Institutions
- University Medical Center Groningen (NL)
- Harvard University (US)
- University of Groningen (NL)
- Mass General Brigham (US)
- Rockefeller University (US)
Publication Details
- Journal
- Mobile DNA
- Published
- 2025-02-06
- DOI
- https://doi.org/10.1186/s13100-024-00339-4
- Citations
- 6
- Primary Topic
- Chromosomal and Genetic Variations
- Type
- article
- Field-Weighted Citation Impact
- 10.50
Funders
- Institute of Mathematical Sciences
- Universitair Medisch Centrum Groningen
- National Institutes of Health
- National Center for Advancing Translational Sciences