Integrated interactome, proteomic and functional analyses reveal molecular pathways driving <scp>L1TD1</scp> ‐induced aggressiveness in <scp>CNS</scp> embryonal tumor cells

L1TD1 is a pluripotency factor required for embryonic stem cell self-renewal; its expression has also been detected in solid tumors, including embryonal tumors of the central nervous system (CNS). Previously, we showed that L1TD1 expression correlates with metastasis formation and shorter overall survival of medulloblastoma patients. Here, we used affinity purification coupled to mass spectrometry to map the L1TD1 interactome, and global proteomics to assess proteins differentially regulated by L1TD1 expression in patient-derived embryonal CNS tumor cell lines. We identified novel L1TD1 interactors and differentially expressed proteins related to cell proliferation, death and motility. Finally, we demonstrated that L1TD1-overexpressing tumor cells have distinct cell morphology with enhanced filopodial formation, higher cell motility, greater proliferation capability, and reduced sensitivity to cisplatin treatment.

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Publication Details

Journal
FEBS Letters
Published
2025-02-06
DOI
https://doi.org/10.1002/1873-3468.70002
Primary Topic
Microtubule and mitosis dynamics
Type
article
Field-Weighted Citation Impact
0.00

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article

Integrated interactome, proteomic and functional analyses reveal molecular pathways driving L1TD1 ‐induced aggressiveness in CNS embryonal tumor cells

Elisa Helena Farias Jandrey, Oswaldo Keith Okamoto, Thiago Mitsugi, Joseph A. Bell et al.
FEBS Letters
Microtubule and mitosis dynamics
article

Integrated interactome, proteomic and functional analyses reveal molecular pathways driving L1TD1 ‐induced aggressiveness in CNS embryonal tumor cells

Elisa Helena Farias Jandrey, Oswaldo Keith Okamoto, Thiago Mitsugi, Joseph A. Bell, Paul Skipp, Rob M. Ewing, Amanda F. Assoni, Matt Sherwood, Brandon Coke
article en

Abstract

L1TD1 is a pluripotency factor required for embryonic stem cell self-renewal; its expression has also been detected in solid tumors, including embryonal tumors of the central nervous system (CNS). Previously, we showed that L1TD1 expression correlates with metastasis formation and shorter overall survival of medulloblastoma patients. Here, we used affinity purification coupled to mass spectrometry to map the L1TD1 interactome, and global proteomics to assess proteins differentially regulated by L1TD1 expression in patient-derived embryonal CNS tumor cell lines. We identified novel L1TD1 interactors and differentially expressed proteins related to cell proliferation, death and motility. Finally, we demonstrated that L1TD1-overexpressing tumor cells have distinct cell morphology with enhanced filopodial formation, higher cell motility, greater proliferation capability, and reduced sensitivity to cisplatin treatment.

FEBS LettersVol. 599(7)
Instituto Biológico (BR), Universidade de São Paulo (BR), University of Southampton (GB)
University of Southampton, Rosetrees Trust, Fundação de Amparo à Pesquisa do Estado de São Paulo, Conselho Nacional de Desenvolvimento Científico e Tecnológico, Universidade de São Paulo, Medical Research Council, Centros de Pesquisa, Inovação e Difusão, Fundação Amazônia Paraense de Amparo à Pesquisa
Good health and well-being
Openalex Percentile: Top 98%
Microtubule and mitosis dynamics
DOISourceOpenAlex
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