Emergence of concurrently transmissible <i>mcr-9</i> and carbapenemase genes in bloodborne colistin-resistant <i>Enterobacter cloacae</i> complex isolated from ICU patients in Kolkata, India

Carbapenem-resistant <i>Enterobacter cloacae</i> complex (CR-ECC) has become a global nosocomial pathogen in last few years. Colistin, the "last resort antibiotic," is being widely used in the treatment of CR-ECC and, consequently, there has been a brisk rise in colistin-resistant CR-ECC isolates. This study was necessitated by the dearth of a comprehensive molecular investigation of colistin-resistant CR-ECC from India. The notorious IncHI2-HI2A plasmid-borne mcr-9 gene along with active acrAB-tolC efflux pump and phoP/Q-pmr A/B two-component system was found to mediate colistin resistance in the study isolates. Interestingly, the mcr-9 gene was also discovered in colistin-sensitive strains and MIC of colistin was found to increase under colistin pressure. Diverse phylogenetic clones along with novel sequence types were detected. This study highlights the necessity for intense monitoring of mcr-9 in conjunction with the existing epidemic clones of CR-ECC strains harboring diverse arrays of transmissible AMR genes.