Comparative assessment of extraction methods and quantitative HPLC estimation of Kaempferol in the leaves of Prosopis Juliflora Linn Growing in Iraq

Kaempferol is an active native flavonoid, has long been recognized for its curative effects. It has been observed to possess numerous pharmaceutical characteristics. High performance liquid chromatography (HPLC) was used as a quantitative evaluation method to determine the best extraction solvent for obtaining Kaempferol from Prosopis juliflora leaves. In this study, ethanol, ethyl acetate and methanol were used as solvents in the extraction of Kaempferol flavonoid from Prosopis juliflora by employing traditional methods of extraction (reflex and maceration). The plant leaves extract of Prosopis juliflora showed existence of Kaempferol compound. Among the three solvents used, it was obvious that ethyl acetate extract of leaves showed the most significant amount of Kaempferol (92.54 mg.L-1). In accordance to the HPLC results, reflex was the most efficient technique for extracting kaempferol from Prosopis juliflora, and ethyl ethanoate was the best solvent. This information may contribute to develop this flavonoid as a possible agent for the prevention and treatment of some diseases