Evaluation of dried blood spot sampling for real-time PCR malaria diagnostics in a rural setting in Angola
The DBS volume used for DNA extraction is a determining factor in the performance of real-time PCR for Plasmodium DNA detection. A DBS volume of 50-100 µl appears to be optimal for malaria diagnosis and Plasmodium species determination by real-time PCR. DBS is a suitable method for sample collection in Cubal followed by real-time PCR analysis in a reference laboratory.
Authors
- Joan Martínez‐Campreciós (ORCID: https://orcid.org/0000-0002-8899-8447)
- Igor Henrique Rodrigues Oliveira (ORCID: https://orcid.org/0000-0001-6018-5797)
- Elena Sulleiro (ORCID: https://orcid.org/0000-0002-9783-6060)
- Sandra Aixut
- Lidia Goterris
- Antonio Muro (ORCID: https://orcid.org/0000-0003-0244-4740)
- Alejandro Mediavilla (ORCID: https://orcid.org/0009-0000-5273-9251)
- Israel Molina (ORCID: https://orcid.org/0000-0001-6642-7515)
- Carles Rubio Maturana (ORCID: https://orcid.org/0000-0002-5615-9278)
- Pedro Fernández‐Soto (ORCID: https://orcid.org/0000-0002-4089-4307)
- María Luisa Aznar
- Beatriz Crego-Vicente (ORCID: https://orcid.org/0000-0002-9560-0492)
- Arlette Nindia
- Begoña Febrer‐Sendra (ORCID: https://orcid.org/0000-0001-5940-6572)
- Aroa Silgado (ORCID: https://orcid.org/0000-0001-7581-0049)
- Patricia Martínez-Vallejo
Institutions
- Universitat Autònoma de Barcelona (ES)
- Universidad de Salamanca (ES)
- Instituto de Salud Carlos III (ES)
- Centro de Investigación Biomédica en Red (ES)
- Vall d'Hebron Hospital Universitari (ES)
- Instituto de Investigación Biomédica de Salamanca (ES)
Publication Details
- Journal
- Parasites & Vectors
- Published
- 2025-02-06
- DOI
- https://doi.org/10.1186/s13071-025-06685-3
- Citations
- 3
- Primary Topic
- Malaria Research and Control
- Type
- article
- Field-Weighted Citation Impact
- 5.64