Protocol to study the impact of breast cancer on colonization resistance of mouse microbiota using network node manipulation
Network analysis is a powerful tool for investigating complex interactions between different microbial taxa within a community. We present a protocol to study the gut microbial community in a mouse model of breast cancer using a network-based approach. Here, we describe the procedures for tumor cell production and inoculation and 16S rRNA data processing. We then detail steps for constructing co-occurrence networks based on correlations between microbial abundances. For complete details on the use and execution of this protocol, please refer to Wu-Chuang et al.<sup>1</sup>.
Authors
- Lourdes Mateos‐Hernández (ORCID: https://orcid.org/0000-0001-9566-7710)
- Alejandro Cabezas‐Cruz (ORCID: https://orcid.org/0000-0002-8660-730X)
- Alejandra Wu‐Chuang
- Janet Avellanet
- Lianet Abuin‐Denis (ORCID: https://orcid.org/0009-0008-1305-3650)
- Dasha Fuentes (ORCID: https://orcid.org/0000-0003-1970-5580)
- Ana Laura Cano‐Argüelles (ORCID: https://orcid.org/0000-0002-1056-8439)
- Apolline Maître (ORCID: https://orcid.org/0000-0002-5586-9569)
- Arlem García
Institutions
- École Nationale Vétérinaire d'Alfort (FR)
- Center of Molecular Immunology (Cuba) (CU)
- University of Havana (CU)
- Institut National de Recherche pour l'Agriculture, l'Alimentation et l'Environnement (FR)
- Systèmes d'Elevage Méditerranéens et Tropicaux - Laboratoire de Recherche sur le Développement de l'Elevage (FR)
- Instituto de Recursos Naturales y Agrobiología de Salamanca (ES)
Publication Details
- Journal
- STAR Protocols
- Published
- 2025-02-06
- DOI
- https://doi.org/10.1016/j.xpro.2025.103618
- Primary Topic
- Gut microbiota and health
- Type
- article
- Field-Weighted Citation Impact
- 0.00
Funders
- European Commission
- European Regional Development Fund