Protocol to study the role of medial entorhinal cortex-basolateral amygdala circuit in context-induced retrieval of morphine withdrawal memory in mice
The medial entorhinal cortex (MEC) is crucial for context memory, yet its role in context-induced retrieval of morphine withdrawal memory remains to be investigated. Here, we present a protocol to evaluate the importance of projection neurons from the MEC to the basolateral amygdala (BLA) (MEC<sup>-BLA</sup> neurons) in mice during context-induced retrieval of morphine withdrawal memory using a conditioned place aversion (CPA) model. We describe steps for surgical procedure and behavioral experiments. Then, we detail procedures of immunofluorescence staining and image analysis. For complete details on the use and execution of this protocol, please refer to Fu et al.<sup>1</sup>.
Authors
- Bin Lai (ORCID: https://orcid.org/0000-0002-1746-056X)
- Ming Chen (ORCID: https://orcid.org/0000-0002-0683-2110)
- Yali Fu
- Ting Ye
Institutions
- Shanghai Medical College of Fudan University (CN)
- Fudan University (CN)
- Zhongshan Hospital (CN)
- Shandong Eye Hospital (CN)
- Shandong First Medical University (CN)
- State Key Laboratory of Medical Neurobiology
Publication Details
- Journal
- STAR Protocols
- Published
- 2025-02-06
- DOI
- https://doi.org/10.1016/j.xpro.2024.103542
- Primary Topic
- Memory and Neural Mechanisms
- Type
- article
- Field-Weighted Citation Impact
- 0.00
Funders
- Natural Science Foundation of Shanghai
- National Natural Science Foundation of China
- Science and Technology Commission of Shanghai Municipality