Synthetically programmed antioxidant delivery by a domesticated skin commensal
Bacteria represent a promising dynamic delivery system for the treatment of disease. In the skin, the relevant location of Cutibacterium acnes within the hair follicle makes this bacterium an attractive chassis for dermal biotechnological applications. Here, we provide a genetic toolbox for the engineering of this traditionally intractable bacterium, including basic gene expression tools, biocontainment strategies, markerless genetic engineering, and dynamic transcriptional regulation. As a proof of concept, we develop an antioxidant-secreting strain capable of reducing oxidative stress in a UV stress model.
Authors
- Nil Campamà Sanz (ORCID: https://orcid.org/0000-0002-7811-5663)
- Guillermo Nevot (ORCID: https://orcid.org/0000-0001-7015-0942)
- Marc Güell (ORCID: https://orcid.org/0000-0003-4000-7912)
- Cristóbal Parra-Cid (ORCID: https://orcid.org/0000-0001-9688-3968)
- Javier Santos‐Moreno (ORCID: https://orcid.org/0000-0001-6268-2623)
- Rodrigo Ledesma‐Amaro (ORCID: https://orcid.org/0000-0003-2631-5898)
- Lorena Toloza (ORCID: https://orcid.org/0000-0001-5442-5352)
- Ellen H. van den Bogaard (ORCID: https://orcid.org/0000-0003-4846-0287)
- Içvara Barbier (ORCID: https://orcid.org/0000-0001-6814-2871)
- Patrick A.M. Jansen (ORCID: https://orcid.org/0000-0002-7894-2140)
Institutions
- Institució Catalana de Recerca i Estudis Avançats (ES)
- Radboud University Nijmegen (NL)
- Universitat Pompeu Fabra (ES)
- Radboud University Medical Center (NL)
- Karolinska Institutet (SE)
- Imperial College London (GB)
Publication Details
- Journal
- Cell Systems
- Published
- 2025-02-01
- DOI
- https://doi.org/10.1016/j.cels.2025.101169
- Citations
- 8
- Primary Topic
- Vitamin C and Antioxidants Research
- Type
- article
- Field-Weighted Citation Impact
- 8.96
Funders
- Office of Naval Research Global
- HORIZON EUROPE European Innovation Council