Genetic Code Expansion for Controlled Surfactin Production in a High Cell-Density Bacillus subtilis Strain
The principle of genetic code expansion enabled a precise control of the surfactin bioproduction as a representative of bioactive secondary metabolites in <i>B. subtilis</i>. This allowed the establishment of inducer-associated regulation at the post-transcriptional level with simultaneous use of the native promoter system. In this way, inductor-dependent control of the production of the target metabolite-forming enzyme could be achieved.
Authors
- Lars Lilge (ORCID: https://orcid.org/0000-0002-7693-477X)
- Oscar P. Kuipers (ORCID: https://orcid.org/0000-0001-5596-7735)
- Philipp Hubel (ORCID: https://orcid.org/0000-0003-3053-3046)
- E Hiller (ORCID: https://orcid.org/0009-0000-3815-920X)
- Rudolf Hausmann (ORCID: https://orcid.org/0000-0002-2327-7120)
- Elvio Henrique Benatto Perino (ORCID: https://orcid.org/0000-0003-0372-260X)
- L. Biermann
- Alexander Hermann
Institutions
- University of Hohenheim (DE)
- University of Groningen (NL)
Publication Details
- Journal
- Microorganisms
- Published
- 2025-02-06
- DOI
- https://doi.org/10.3390/microorganisms13020353
- Citations
- 5
- Primary Topic
- Bacterial Genetics and Biotechnology
- Type
- article
- Field-Weighted Citation Impact
- 9.09
Funders
- Deutsche Forschungsgemeinschaft
- Bundesministerium für Bildung und Forschung
- Jiangnan University