Protocol for the intracerebral injection of AAV-α-synuclein plus poly(ADP-ribose) to generate a Parkinson’s disease monkey model
The lack of an etiologic Parkinson's disease (PD) model in non-human primates has inhibited our understanding of the disease and the development of therapies. Here, we present a protocol for generating a PD model in Macaca fascicularis. We describe steps for localizing the substantia nigra and striatum using magnetic resonance imaging (MRI). We then detail a technique for the intracerebral administration of adeno-associated virus (AAV)-mediated overexpression of α-synuclein into the substantia nigra plus the injection of poly(ADP-ribose) (PAR) into the striatum. For complete details on the use and execution of this protocol, please refer to Liu et al.<sup>1</sup>.
Authors
- Shuyi Liu (ORCID: https://orcid.org/0000-0002-2065-156X)
- Wei Si
- Zhengbo Wang (ORCID: https://orcid.org/0000-0002-4717-5137)
Institutions
- Kunming University of Science and Technology (CN)
- Yunnan Yingmao Biotechnology (China) (CN)
Publication Details
- Journal
- STAR Protocols
- Published
- 2025-02-06
- DOI
- https://doi.org/10.1016/j.xpro.2025.103621
- Primary Topic
- Parkinson's Disease Mechanisms and Treatments
- Type
- article
- Field-Weighted Citation Impact
- 0.00
Funders
- National Natural Science Foundation of China
- Natural Science Foundation of Yunnan Province
- National Key Research and Development Program of China
- Cultivating Plan Program for the Leader in Science and Technology of Yunnan Province
- National Key Research and Development Program of China Stem Cell and Translational Research