Single cell RNA sequencing of haematopoietic cells in fresh and frozen human atheroma tissue
Our study demonstrates that cryopreserved human atheroma samples can be successfully profiled using scRNA-seq, with comparable transcriptomic data to that obtained from fresh samples. These findings suggest that cryopreservation is a viable method for biobanking atheroma tissues, facilitating large-scale studies without the need for immediate sample processing.
Authors
- Robyn Fong (ORCID: https://orcid.org/0000-0002-7953-5109)
- Patricia K. Nguyen (ORCID: https://orcid.org/0000-0001-7170-9787)
- Xianxi Huang (ORCID: https://orcid.org/0000-0001-5978-5031)
- Herra Ahmad (ORCID: https://orcid.org/0000-0003-1871-9614)
- Jayakrishnan Gopakumar (ORCID: https://orcid.org/0000-0003-3895-8188)
- Tiffany Koyano (ORCID: https://orcid.org/0000-0002-7646-0623)
- Jack Boyd (ORCID: https://orcid.org/0000-0002-3020-5280)
- Y. Joseph Woo (ORCID: https://orcid.org/0000-0002-2506-492X)
- Daniel Nachun (ORCID: https://orcid.org/0000-0002-0271-1135)
- Jessica D’Addabbo (ORCID: https://orcid.org/0000-0001-9562-2117)
- Siddhartha Jaiswal (ORCID: https://orcid.org/0000-0002-9597-0477)
- Oliver Aalami (ORCID: https://orcid.org/0000-0002-7799-2429)
- Lisa Ma
Institutions
- California Institute for Regenerative Medicine (US)
- VA Palo Alto Health Care System (US)
- Cardiovascular Institute of the South (US)
- Shantou University (CN)
- Palo Alto Veterans Institute for Research (US)
- First Affiliated Hospital of Shantou University Medical College (CN)
- Stanford Medicine (US)
- Charité - Universitätsmedizin Berlin (DE)
- Stanford University (US)
Publication Details
- Journal
- Cardiovascular Research
- Published
- 2025-02-01
- DOI
- https://doi.org/10.1093/cvr/cvaf014
- Citations
- 3
- Primary Topic
- Single-cell and spatial transcriptomics
- Type
- article
- Field-Weighted Citation Impact
- 2.42