Single-chain variable fragment affinity tuning can optimize anti-AML CAR-NK cell functionality
Expression of 26292 and 7G3 CARs with a range of CD123 binding affinities in NK cells leads to antigen-specific activation and cytotoxicity against AML. Affinity-based differences in functional activation and antitumor activity are dependent on time course and are scFv/epitope specific.
Authors
- Jun Choe (ORCID: https://orcid.org/0000-0002-3389-5969)
- Danielle Jones (ORCID: https://orcid.org/0000-0003-2773-4293)
- Ilias Christodoulou (ORCID: https://orcid.org/0000-0002-3527-1834)
- Ruyan Rahnama (ORCID: https://orcid.org/0000-0003-3025-3546)
- Monika Kizerwetter (ORCID: https://orcid.org/0000-0001-6084-7471)
- Challice Lee Bonifant (ORCID: https://orcid.org/0000-0001-9028-1683)
- Marianna Zahurak (ORCID: https://orcid.org/0000-0002-5086-6721)
- Stamatia C. Vorri (ORCID: https://orcid.org/0000-0002-6100-1985)
- Natalie J. Holl (ORCID: https://orcid.org/0000-0001-8503-0839)
- Ravi Varadhan (ORCID: https://orcid.org/0000-0002-8434-1034)
- Yun‐Huai Kuo (ORCID: https://orcid.org/0000-0001-5994-117X)
- Jamie B. Spangler (ORCID: https://orcid.org/0000-0001-8187-3732)
- N. Espinoza
- Megan Zinsky
- Yang Huilin
- Christian Guaraca
Institutions
- Bloomberg (United States) (US)
- Johns Hopkins University (US)
- Johns Hopkins Medicine (US)
- Sidney Kimmel Comprehensive Cancer Center (US)
Publication Details
- Journal
- Journal for ImmunoTherapy of Cancer
- Published
- 2025-02-01
- DOI
- https://doi.org/10.1136/jitc-2024-010763
- Citations
- 8
- Primary Topic
- Immune Cell Function and Interaction
- Type
- article
- Field-Weighted Citation Impact
- 6.38
Funders
- National Science Foundation
- Alex's Lemonade Stand Foundation for Childhood Cancer
- St. Baldrick's Foundation
- Johns Hopkins University
- Gabrielle's Angel Foundation for Cancer Research
- Allegheny Health Network
- National Institutes of Health