Comparison of air-liquid interface transwell and airway organoid models for human respiratory virus infection studies
In conclusion, all three models produced consistent results regardless of the varying cell sources, culturing approaches, and infection methods. Our collaborative efforts to harmonize infection experiments and compare ALI transwell and airway organoid models described here aid in advancing our understanding and improving the standardization of these complex <i>in vitro</i> respiratory models for future studies.
Authors
- Fan Zhou (ORCID: https://orcid.org/0000-0001-9918-3422)
- Cécile A. C. M. van (ORCID: https://orcid.org/0000-0003-1995-8641)
- Anke Huckriede (ORCID: https://orcid.org/0000-0002-2646-5143)
- Rosanne W. Koutstaal
- Puck B. van Kasteren (ORCID: https://orcid.org/0000-0003-4369-6055)
- Agnete S. T. Engelsen (ORCID: https://orcid.org/0000-0003-0375-3406)
- Joanna Waldock (ORCID: https://orcid.org/0000-0002-3622-5899)
- Nathalie Mantel (ORCID: https://orcid.org/0000-0001-8984-3183)
- Nilima Dinesh Kumar (ORCID: https://orcid.org/0000-0003-2788-0303)
- Karl A. Brokstad (ORCID: https://orcid.org/0000-0003-0593-2731)
- Kevin Buno
- Simon P. Jochems (ORCID: https://orcid.org/0000-0002-4835-1032)
- Rebecca Jane Cox (ORCID: https://orcid.org/0000-0002-8341-4078)
- Othmar G. Engelhardt (ORCID: https://orcid.org/0000-0002-0465-9908)
- Camilla T. Ekanger
- Martin Beukema (ORCID: https://orcid.org/0000-0002-8746-817X)
- Barbro N. Melgert (ORCID: https://orcid.org/0000-0001-7091-907X)
- Niels L. Mulder
Institutions
- University Medical Center Groningen (NL)
- University of Groningen (NL)
- Western Norway University of Applied Sciences (NO)
- Utrecht University (NL)
- Leiden University Medical Center (NL)
- Haukeland University Hospital (NO)
- Sanofi (France) (FR)
- National Institute for Public Health and the Environment (NL)
- GlaxoSmithKline (Italy) (IT)
- University of Bergen (NO)
Publication Details
- Journal
- Frontiers in Immunology
- Published
- 2025-02-06
- DOI
- https://doi.org/10.3389/fimmu.2025.1532144
- Citations
- 14
- Primary Topic
- Inhalation and Respiratory Drug Delivery
- Type
- article
- Field-Weighted Citation Impact
- 21.26
Funders
- European Federation of Pharmaceutical Industries and Associations
- European Commission
- Innovative Medicines Initiative