Tackling somatic DNA contamination in sperm epigenetic studies
The comparison of Infinium Human Methylation 450K BeadChip data for sperm and blood samples identified 9564 CpG sites that can be used as markers for analyzing somatic DNA contamination. We have put together a comprehensive plan including evaluation under a microscope, SCLB treatment, inclusion of CpG biomarkers for sample quality evaluation, and applying a 15% cut off at the time of data analysis to completely eliminate the influence of somatic DNA contamination in sperm epigenetic studies. We conclude that if this comprehensive plan is followed, the influence of somatic DNA contamination in sperm epigenetic studies can be completely eliminated.
Authors
- Singh Rajender (ORCID: https://orcid.org/0000-0002-4592-6566)
- Anamika Kumari (ORCID: https://orcid.org/0000-0001-7411-7920)
Institutions
- Central Drug Research Institute (IN)
- Academy of Scientific and Innovative Research (IN)
Publication Details
- Journal
- Frontiers in Reproductive Health
- Published
- 2025-02-05
- DOI
- https://doi.org/10.3389/frph.2025.1506117
- Primary Topic
- Epigenetics and DNA Methylation
- Type
- article
- Field-Weighted Citation Impact
- 0.00