Development of recombinase polymerase amplification combined with a lateral flow dipstick assay for rapid and simple detection of <scp> <i>Tylenchulus semipenetrans</i> </scp> in soil
The developed RPA-LFD assay is a simple, rapid, specific, sensitive and visual method for detecting T. semipenetrans. It shows great potential for on-site rapid detection applications, especially in resource-limited settings. © 2025 Society of Chemical Industry.
Authors
- Caisheng Qiu
- Qipei Wang
- Tuhong Wang (ORCID: https://orcid.org/0000-0002-7442-2877)
- Yi Cheng
- Huajiao Qiu (ORCID: https://orcid.org/0000-0001-7610-7275)
- Zhiqiang Song (ORCID: https://orcid.org/0009-0004-9003-2715)
Institutions
- Institute of Bast Fiber Crops (CN)
- Chinese Academy of Agricultural Sciences (CN)
Publication Details
- Journal
- Pest Management Science
- Published
- 2025-02-05
- DOI
- https://doi.org/10.1002/ps.8693
- Citations
- 1
- Primary Topic
- Nematode management and characterization studies
- Type
- article
- Field-Weighted Citation Impact
- 1.75
Funders
- National Natural Science Foundation of China
- Natural Science Foundation of Hunan Province