<i>In Vivo</i> Study and Cytotoxicity of Migrated Silver Nanoparticles (AgNPs) from Cellulose/LDPE/AgNP Nanocomposite in Highly Perishable Food (Fish Fillet) Packaging

A small number of studies have been carried out since the beginning of the proposal and construction of nanocomposite packaging to minimize the migration of nanomaterials, along with the precise determination of the migration rate to one of the most perishable food items (fish fillet), examining the cytotoxicity level, the impact of migrated nanomaterials on the oral microbiota, and the absorption of the nanomaterials by various organs. All of these are the main focus of this study. This study proposes a composite with a cellulose and low-density polyethylene (LDPE) matrix to reduce the amount of nanosilver migration. In addition to its suitable mechanical properties, this nanocomposite has a meager amount of nanosilver migration. According to ICP-MS measurements, the maximum amount of nanosilver migrated to fish fillets was 1.85 μg/mL. The amount of nanosilver that migrated was administered orally by gavage for 10 days to Wistar rats. The results did not show the absorption of nanosilver in various organs (liver, kidney, small intestine, and the blood).