Characterization of Begomovirus Isolates Causing YMD in soybean and Analyzing the Relation with Early Reported Strains of Begomovirus from New Delhi Region

This work was focused on the exact characterization of begomovirus correlated with yellow mosaic disease (YMD) in soybean during the monsoon season. This information helped to understand the time-bound status of the viral group. Augmented design and was carried out at the research field. Starting material source for pure viral DNA Initial source material consisted of soybean plants (JS335) with a prominent yellow mosaic symptom that were grown in sick plots under controlled conditions in order to obtain a DNA samples that also contained the genomes of begomovirus. The experiment used an augmented design and was carried out at the research field in New Delhi during the 2018 kharif season. In each replication, the susceptible cultivar (JS335) genotypes were planted for the establishment of a sick soybean plot for YMD development purposes. A limited number of MYMIV DNA-A sequences was used to create the phylogenetic tree. Cluster W was used to align the sequences, with the default gap penalty values of gap opening 10 and extension 0.2. A neighbor-joining tree was built using MEGA 11.0, a p-distance model, and pairwise gap deletion. The viral genome was amplified and sequenced. The sequence analysis revealed that the viral genome is 2747 nucleotides long, similar to monopartite begomoviruses, and has seven conserved ORFs. Further nucleotide (nts) sequence comparisons revealed that the genome had the maximum sequence identity of 99%, which is identical to the previously reported sequence. The DNA-A of MYMIV (GenBank accession number: OQ473638) was made from 2747 nucleotides that were 99% the same as the sequence that had already been reported. Previously, that Mungbean yellow mosaic virus (MYMV) and Mungbean yellow mosaic India virus (MYMIV) are the primary begomoviruses causing yellow mosaic disease (YMD) in soybeans in India. This study also demonstrated that our isolate of MYMIV was more similar to the one that was already recognized to be Mu2 New Delhi isolate. This information would be useful in more detailed screening processes under controlled condition and evaluation of soybean genotypes as part of variety development for sustainable cultivation of soybean.