Contactless Bioprinting Based on Acoustic Tweezer

3D bioprinting technology presents an expansive array of possibilities for tissue engineering, drug screening, and foundational research. However, its operational demands necessitate a meticulous balance between pollution prevention and minimizing cellular harm. Traditional inkjet and extrusion bioprinting methods lead to direct nozzle-to-object contact, heightening the risk of contamination and cellular injury. To address these challenges, we propose a novel bioprinting approach harnessing acoustic tweezer in conjunction with a three-degree-of-freedom displacement platform. Herein, hydrogel droplets containing cells are trapped by acoustic tweezer and subsequently transported and positioned at the desired location via the displacement platform. This innovative technique avoids direct nozzle contact with the printed object, thus offering a viable strategy for mitigating contamination and minimizing cellular damage throughout the printing process.