Microfluidic Formation of Porous Microgels with Interior Spiral Canals for Mesenchymal Stem Cell Culture

Stem cell delivery is pivotal in tissue engineering, enabling the regeneration and repair of damaged tissues and advancing therapeutic solutions. As an innovative stem cell delivery system, microgels provide a supportive, protective environment that enhances cell viability and promotes targeted tissue regeneration. However, conventional microgels face limitations due to the lack of microporous structures and interior canals, which hinder efficient nutrient and oxygen exchange, thereby reducing cell viability and proliferation. To overcome these challenges, we developed a droplet microfluidic system for the stable and continuous production of porous microgels with interior spiral canals by introducing a pore-forming agent. This controlled formation process results in microgels with excellent porosity and monodispersity. With their unique structure and biocompatibility, these porous microgels are excellent microcarriers for 3D cell culture, enhancing nutrient and oxygen exchange and providing a conducive environment for cell growth and proliferation.