A protocol for assessing the adhesion potential of lactic acid bacteria on various cell types
Lactic acid bacteria (LAB) are a large group produced during carbohydrate fermentation, resulting in lactic acid. Here, we present a protocol for probiotic adhesion and interaction into buccal epithelial cells, chicken epithelial cells, and HT-29 cells, as well as for autoaggregation and coaggregation. We describe steps for visualizing LAB adhesion by imaging cell adhesion using a light microscope. We then detail procedures for performing an adhesion assay. For complete details on the use and execution of this protocol, please refer to Kumari et al.<sup>1</sup>.
Authors
- Khang Wen Goh (ORCID: https://orcid.org/0000-0001-6686-7019)
- V. Kumari (ORCID: https://orcid.org/0000-0003-1806-8452)
- Lata Jayaram (ORCID: https://orcid.org/0000-0002-6656-6045)
- Ramith Ramu (ORCID: https://orcid.org/0000-0003-2776-5815)
- M. K. Jayanthi
- Deepthi Puttegowda
Institutions
- INTI International University (MY)
- JSS Academy of Higher Education and Research (IN)
Publication Details
- Journal
- STAR Protocols
- Published
- 2025-02-05
- DOI
- https://doi.org/10.1016/j.xpro.2025.103619
- Citations
- 6
- Primary Topic
- Probiotics and Fermented Foods
- Type
- article
- Field-Weighted Citation Impact
- 6.97
Funders
- JSS Academy of Higher Education and Research