Modulation Of Coagulase-Negative Staphylococcus Strains By Lippia Origanoides Essential Oil Associated With BetaLactam Antibiotics

Background: Coagulase-Negative Staphylococcus (CoNS) can cause infection at the primary colonization site or spread to cause serious hospital infections such as bacteremia, septicemia and neonatal sepsis. In this context, the increase in bacterial resistance to conventional antibiotics has become a public health problem. Therefore, there is a need to intensify studies involving CoNS to implement more effective control measures. In this context, essential oils, which have antimicrobial activity, have emerged as alternatives for modulating the activity of antimicrobial-resistant bacterial strains. This study evaluated the modulatory effect of rosemary-pepper essential oil associated with antibiotics against CoNS strains. Materials and Methods: Homogeneous populations of pepper rosemary were collected around CPCA/UFMG. Characterization was obtained by gas chromatography. The microorganisms were identified by MALDI - TOF MS and antibiotic resistance was determined by microdilution on plates. The STITCH bioinformatics platform was used to predict the potentiation of antibiotics through rosemary essential oil. Results: Carvacrol (28.94%), o-Cymene (20.53%), γ-Terpinene (10.36%) and Thymol (2.91%) were obtained as the majority components of the oil. The association of pepper rosemary oil with the antibiotics amoxicillin and tetracycline reduced the concentration of antibiotic needed to inhibit 100% of the resistant strains of CoNS, indicating the synergistic effect of the oil with the antibiotics. In the networks and sub-networks created by the in silico analyses, it is possible to observe an interaction between the antibiotic amoxicillin and the bioactive compound in pepper rosemary oil, Camphor, through the histamine molecule. The antibiotic tetracycline was found to interact with the bioactive compounds in rosemary oil, linalool and limonene, via the nitric oxide synthase 3 (NOS3) molecule. All the major components of the oil also acted in the chemical-protein interaction network, indicating possible synergisms between the antibiotics and the bioactive compounds in rosemary oil. Conclusion: These results show that pepper rosemary oil has the potential to be used as an antimicrobial adjuvant in the treatment of infections caused by antibiotic-resistant CoNS bacteria