Validation of an Analytical Method of Hyperoside and Quercetin-3-glucoside for the Standardization of Water Dropwort (Oenanthe javanica DC.) Extract as a Functional Ingredient

In this study, we developed and validated a high-performance liquid chromatography/diode array method for the quantification of Hyperoside and quercetin-3-glucoside as a marker compound in water dropworth (Oenanthe javanica DC.) extract (WDE). Method validation was performed by measuring specificity, linearity, limit of detection (LOD), limit of quantitation (LOQ), accuracy, and precision. Hyperoside and quercetin-3-glucoside were separated without interference from other substances at the same retention time and spectrum as the standard solution, demonstrating the analytical specificity. Calibration curves of hyperoside and quercetin-3-glucoside exhibited high linearity (R² ≥1.000). The LOD and LOQ of hyperoside and quercetin-3-glucoside were 0.16, 0.49 μg/mL and 0.14, 0.41 μg/mL, respectively. The recovery rate of hyperoside and quercetin-3-glucoside were obtained 97.20–99.78% and 99.44–104.37%, respectively, indicating that the results were highly accuracy. Relative standard deviation values for precision of hyperoside and quercetin-3-glucoside were 0.84-1.62% and 1.96-2.50%, respectively. These results indicate that the proposed HPLC/DAD method for the detection of hyperoside and quercetin-3-glucoside in WDE is simple, reliable, and reproducible. Therefore, this analytical method could be used as basic data for the development of functional ingredients for health functional food of WDE.