Finding the best column for polar basic analytes across reversed-phase and hydrophilic interaction liquid chromatography
A straightforward comparison and ranking system is developed to test multiple chromatographic columns with different stationary phases, from reversed-phase to hydrophilic interaction in gradient elution, seeking the best method for separating polar basic molecules. Nine columns were compared in multiple conditions. The best setup resulting in the competition is presented in detail, which can be applied to a wide range of analytes.
Authors
- Magdolna Kovács
- Pál Tamás Szabó (ORCID: https://orcid.org/0000-0003-2260-4641)
- József Simon (ORCID: https://orcid.org/0000-0003-1174-4702)
Institutions
- University of Pannonia (HU)
- Budapest University of Technology and Economics (HU)
- HUN-REN Research Centre for Natural Sciences (HU)
Publication Details
- Journal
- Heliyon
- Published
- 2025-02-01
- DOI
- https://doi.org/10.1016/j.heliyon.2025.e42461
- Citations
- 1
- Primary Topic
- Analytical Chemistry and Chromatography
- Type
- article
- Field-Weighted Citation Impact
- 0.76
Funders
- Nemzeti Kutatási és Technológiai Hivatal
- Nemzeti Kutatási Fejlesztési és Innovációs Hivatal
- National Research, Development and Innovation Office