Salinity induced changes in esterase, peroxidase and alcohol dehydrogenase isozymes and leaf soluble proteins in salinity susceptible and salinity tolerant sugarcane genotypes
The salinity susceptible CoC-671 and salinity tolerant sugarcane genotype CoM-265 were evaluated for Peroxidase (POX), Esterase (EST) and Alcohol Dehydrogenase (ADH) isozymes and soluble protein profiling by SDS and native-PAGE at salinity levels 0.41 dSm<sup>-1</sup>, 2.31 dSm<sup>-1</sup>, 4.21 dSm<sup>-1</sup>, and 8.01 dSm<sup>-1</sup> maintained by NaCl solution. The plant height, number of leaves and seedling diameter got reduced in salinity susceptible sugarcane genotype CoC-671 as well as salinity tolerant sugarcane genotype CoM-265 with increase in salinity levels. However, reduction in plant height, number of leaves and seedling diameter was less in salinity tolerant sugarcane genotype CoM-265 as compared to salinity susceptible sugarcane genotype CoC-671. The POX isozyme profiling revealed that salinity susceptible CoC-671 and salinity tolerant sugarcane genotype CoM-265 had variation in soluble protein band intensity at different salinity levels with relative mobility (Rm) 0.137. The present study could be useful for genetic variability analysis in sugarcane genotypes differing in salinity stress tolerance capability.
Authors
- R D Satbhai
- Shashikala R. Inamdar (ORCID: https://orcid.org/0000-0003-1999-1900)
- M. R. Patil (ORCID: https://orcid.org/0000-0002-8628-5734)
- Parashram Patil (ORCID: https://orcid.org/0000-0002-9860-8166)
- AA Kale
- Ajay Kumar Singh (ORCID: https://orcid.org/0000-0002-4533-8705)
Institutions
- Sant Gadge Baba Amravati University (IN)
- Mahatma Phule Krishi Vidyapeeth (IN)
- National Institute of Abiotic Stress Management (IN)
Publication Details
- Journal
- Biotechnology Reports
- Published
- 2025-02-05
- DOI
- https://doi.org/10.1016/j.btre.2025.e00880
- Primary Topic
- Plant responses to water stress
- Type
- article
- Field-Weighted Citation Impact
- 0.00