Mos2 2D nanoparticles as inducers of changes in liposomes formation and their spectroscopic properties

Liposomes, due to their biocompatible and flexible lipid bilayer, serve as ideal model structures to study the biophysical properties and physiological functions of cell membranes. To investigate the impact of 2D nanoparticles on liposomes formation, methods of dynamic light scattering, optical and SEM microscopy were used to determine the size of liposomes, along with Raman, IR spectroscopy and quantum-chemistry calculations for structural study. Optical microscopy study confirmed feasibility of the preparation method and liposomes size redistribution, depending on the nanoparticles. SEM data and EDS analysis provided insides on the morphological structures of the nanopowder, nanoplatelets, liposomes, and its elemental composition. Sizes of the liposomes increase up to 12% with addition of 2D MoS2 nanoparticles. Analysis of IR spectra showed a change in the spectral properties of liposomes with particles in comparison with unloaded liposomes in the region of stretching C = O and CH vibration, bending OH. Changes in H-bonds redistribution, contribution from = C–H stretching ring vibrations at 3035 cm–1 and disappearance of the band at 3006 cm–1 in IR spectrum together with the virtual disappearance of Raman bands of liposomes seems to be a marker for particle entry into the liposome. Quenching of luminescence in Raman spectra is observed in liposomes with nanoparticles compared to unloaded liposomes which is weaker in the case of liposome preparation together with nanoparticles. Enhancement of intensity of vibration modes in IR spectra for nanoparticles introduced after liposome preparation from 34% (powder) to 52% (platelets) and covered the liposome surface is observed and could be liposome particle coating marker.