Antimicrobial peptide DiPGLa-H exhibits the most outstanding anti-infective activity among the PGLa variants based on a systematic comparison
AMPs are innate defense molecules in animals, plants, and microorganisms. Notably, one-third of these peptides in databases originate from amphibians. We discovered that naturally weak AMPs from this source can be enhanced through artificial design. Specifically, variant DiPGLa-H showed superior germicidal efficacy and cell selectivity both <i>in vivo</i> and <i>in vitro</i> and can be biosynthesized and purified by combining DAMP4 fusion protein strategy and a simple non-chromatographic method that facilitates large-scale production. Our focus is on understanding the structure-activity relationships of PGLa. Furthermore, the development of a non-chromatographic purification technique for AMPs offers a viable pathway for the large-scale production of these essential compounds.
Authors
- Fatma A. El-Gohary (ORCID: https://orcid.org/0000-0001-5883-2967)
- Pilong Liu (ORCID: https://orcid.org/0000-0003-1981-5341)
- Chen Wang (ORCID: https://orcid.org/0000-0003-4055-7391)
- Huping Xue (ORCID: https://orcid.org/0000-0002-7808-7164)
- Yadong Ma
- Muhammad Zafir
- Xuemei Xue
- Xin Zhao (ORCID: https://orcid.org/0000-0002-1078-7332)
- Ziqian Zhang (ORCID: https://orcid.org/0000-0001-9294-660X)
- Xiaokun Wang (ORCID: https://orcid.org/0000-0003-4148-5561)
- Ping Li (ORCID: https://orcid.org/0000-0002-8515-7773)
- Fuyuan Yang (ORCID: https://orcid.org/0000-0001-5389-4229)
- Liangjun Zheng (ORCID: https://orcid.org/0009-0009-3655-8683)
Institutions
- Mansoura University (EG)
- McGill University (CA)
- Northwest A&F University (CN)
Publication Details
- Journal
- Applied and Environmental Microbiology
- Published
- 2025-02-05
- DOI
- https://doi.org/10.1128/aem.02062-24
- Primary Topic
- Antimicrobial Peptides and Activities
- Type
- article
- Field-Weighted Citation Impact
- 0.00
Funders
- National Natural Science Foundation of China
- Sichuan Agricultural University