Study on the Anti-Aging Effect of Polygonatum Cyrtonema Hua on Human Skin Fibroblasts

Background: Polygonatum cyrtonema Hua (PCH) is a traditional Chinese medicine, a plant with the same origin of medicine and food, and has high edible value. However, whether Polygonatum cyrtonema Hua has skin care efficacy remains to be studied. Aims: To explore the anti-aging effect of Polygonatum cyrtonema Hua extract on human skin fibroblasts (FB). Materials and Methods: An aging model of FB cells induced by H2O2 plus passage was established in vitro. The effect of PCH extract on β - galactosidase, a key index of cell aging, was detected by staining method; The quantitative real-time polymerase chain reaction (qRT-PCR) was used to detect the mRNA expression level of p21 in cells treated with PCH extract; ELISA was used to detect the effects of IL-6, IL-8 inflammatory factors, matrix metalloproteinase-1 (MMP-1) and MCP chemokines in senescent cells; Immunofluorescence detection of DNA damage related protein γH2A.X in cells treated with PCH extract. Results: Compared with the blank control group, PCH extract can reduce the activity of β - galactosidase in FB cells, reduce the mRNA expression level of p21, inhibit the content of IL-6, IL-8, MMP1, MCP, and inhibit the expression of γH2A.X. Conclusions ：PCH extract can fight against cell aging by reducing SASPs related indicators after cell aging, and can be used as a potential natural active substance for skin anti (inflammatory) aging.