Enhancement of Semen Cryopreservation from Native Thai Bulls Through Moringa oleifera Leaf Extract Supplementation

Cryopreservation of native Thai bull semen often results in significant post-thaw quality reduction, underscoring the need for effective cryoprotective strategies. This study investigated the effect of <i>Moringa oleifera</i> leaf extract (MOLE) as an antioxidant supplementation by incorporating four MOLE concentrations (0-1.5% [<i>w</i>/<i>v</i>]) into a standard semen extender, followed by cryopreservation using liquid nitrogen vapor freezing. Data were analyzed using a randomized complete block design with Tukey's post hoc test (<i>p</i> < 0.05). Post-thaw analysis of semen revealed that 1 mg/mL MOLE significantly enhanced total sperm motility, progressive sperm motility, sperm viability, and sperm plasma membrane integrity compared to the control and other MOLE concentrations (<i>p</i> < 0.05). This concentration also improved the amplitude of lateral head displacement and curvilinear velocity and reduced malondialdehyde levels in semen samples (<i>p</i> < 0.05), indicating reduced lipid peroxidation. Higher MOLE concentrations negatively impacted semen quality. In conclusion, supplementation with 1 mg/mL MOLE markedly improved post-thaw semen quality and reduced lipid peroxidation, suggesting its potential as an antioxidant for enhancing reproductive outcomes in native Thai bulls.