Detection and subtyping of Blastocystis sp. in human and animal stool samples using high-resolution melting analysis
ST3 (28%) and ST7 (30%) were the most prevalent subtypes across all samples, with distinct distributions in human and animal hosts. The HRM technique demonstrated efficiency and cost-effectiveness, providing a rapid and accurate method for Blastocystis subtype identification in developing countries, which can expedite diagnostic responses and reduce the need for sequencing. This supports the potential for HRM to improve epidemiological surveillance and understanding of cross-species transmission.
Authors
- Mehdi Zarean (ORCID: https://orcid.org/0000-0003-1138-1147)
- Elham Moghaddas (ORCID: https://orcid.org/0000-0002-7080-2768)
- Fariba Berenji (ORCID: https://orcid.org/0000-0002-8412-8858)
- Bibi Razieh Hosseini Farash (ORCID: https://orcid.org/0000-0003-2748-9435)
- Lida Jarahi (ORCID: https://orcid.org/0000-0001-7753-6610)
- Ghodratollah Salehi Sangani (ORCID: https://orcid.org/0000-0003-0451-7755)
- Shaghayegh Moghadam (ORCID: https://orcid.org/0000-0002-9694-2873)
- Ahmad Hosseini-Safa
- Pooria Salehi Sangani
- Ali Gholizadeh (ORCID: https://orcid.org/0000-0002-2310-0544)
Institutions
- Iran University of Medical Sciences (IR)
- Mashhad University of Medical Sciences (IR)
- Gonabad University of Medical Sciences (IR)
Publication Details
- Journal
- BMC Infectious Diseases
- Published
- 2025-02-05
- DOI
- https://doi.org/10.1186/s12879-024-10423-y
- Citations
- 4
- Primary Topic
- Parasitic Infections and Diagnostics
- Type
- article
- Field-Weighted Citation Impact
- 8.19
Funders
- Mashhad University of Medical Sciences