A ribodepletion and tagging protocol to multiplex samples for RNA-seq based virus detection: application to the cassava virome
The use of the Ribo-M-Seq protocol will pave the way for large-scale sample analyses of collections with potentially complex viromes, such as those collected in the West African cassava integrated pest management program.
Authors
- Pierre Lefeuvre (ORCID: https://orcid.org/0000-0003-2645-8098)
- Justin S. Pita (ORCID: https://orcid.org/0000-0003-2815-5904)
- Fidèle Tiendrébéogo (ORCID: https://orcid.org/0000-0002-3619-3268)
- Daniel H. Otron (ORCID: https://orcid.org/0009-0009-4236-5212)
- Jean-Michel Lett (ORCID: https://orcid.org/0000-0003-2013-0016)
- Murielle Hoareau
Institutions
- Centre de Coopération Internationale en Recherche Agronomique pour le Développement (FR)
- Can Tho University (VN)
- Peuplements végétaux et bioagresseurs en milieu tropical (RE)
- Université Félix Houphouët-Boigny (CI)
Publication Details
- Journal
- Virology Journal
- Published
- 2025-02-05
- DOI
- https://doi.org/10.1186/s12985-025-02634-9
- Primary Topic
- Plant Virus Research Studies
- Type
- article
- Field-Weighted Citation Impact
- 0.00
Funders
- Bill and Melinda Gates Foundation
- Centre de Coopération Internationale en Recherche Agronomique pour le Développement
- Conseil Régional de La Réunion
- Foreign, Commonwealth and Development Office
- European Regional Development Fund