Differential Attraction of Summer and Winter Morphs of Spotted Wing Drosophila, Drosophila suzukii, to Yeasts

Drosophila suzukii is a pest of soft and stone fruits that is attracted to yeast volatile metabolites. Drosophila suzukii has distinct summer and winter morphs which are found in different habitats. Complex communities of yeasts likely differ between habitats and thus we hypothesized morphs differ in their attraction to yeast volatiles from different species and combinations of species. We presented D. suzukii with yeast species in isolation and in combinations, as either post-culture mixes or co-cultured, and measured activity of the flies in laboratory choice tests with a Locomotor Activity Monitor as a proxy for attraction. Candida zemplinina was more attractive to winter than summer morphs when cultured in both sterile strawberry juice (SSJ) and artificial culture media (YPD). No significant correlations were found between principle component scores derived from single yeast volatile profiles and fly activity. There was also differential attraction of morphs to certain yeast mixes, most notably post SSJ culture mixes of Hanseniaspora uvarum and C. zemplinina were consistently more attractive to winter morphs. Mixtures of Metschnikowia pulcherrima + Pichia pijperi + H. uvarum in SSJ and M. pulcherrima + P. pijperi yeasts in YPD were also preferred by winter morphs, whilst summer morphs were attracted to SSJ co-cultures of M. pulcherrima + H. uvarum. However, co-culturing yeasts did not enhance attraction compared to post-culture mixes of constituent yeasts for either morph. Differential attraction of morphs to certain yeasts suggests options for new bait formulations in integrated pest management strategies may be tailored to better target morphs.