Identification and Characterization of Two Se6OMTs from Stephania epigaea Offer Novel Insights into the Biosynthetic Pathway of Cepharanthine
This study focused on 6OMT enzymes in <i>S. epigaea</i>, identifying Se6OMTs involved in the cepharanthine biosynthetic pathway, determining the OMTs involved in the production of (<i>R</i>)- and (<i>S</i>)-stereoisomer BIAs. This research provides valuable insights into the substrate promiscuity of Se6OMTs on (<i>R</i>)- and (<i>S</i>)-configured 1-BIAs in <i>S. epigaea</i> and highlights the genetic components necessary for the metabolic engineering and synthetic biology approaches to cepharanthine production.
Authors
- Juan Guo (ORCID: https://orcid.org/0000-0002-1327-4428)
- Julian Wei-Tze Tang (ORCID: https://orcid.org/0000-0002-4963-1028)
- Xinyi Li (ORCID: https://orcid.org/0009-0003-1144-5363)
- Qishuang Li (ORCID: https://orcid.org/0000-0002-2148-7917)
- Jian Wang (ORCID: https://orcid.org/0000-0002-9386-7986)
- Xiaohui Ma (ORCID: https://orcid.org/0000-0001-5961-3168)
- Ying Ma (ORCID: https://orcid.org/0000-0002-4247-3354)
- Jingyi Gan
- Shukun Gong
- Wenlong Shi
- Xingyu Zhao (ORCID: https://orcid.org/0009-0008-3213-0295)
Institutions
- Yunnan University (CN)
- Chinese Academy of Medical Sciences & Peking Union Medical College (CN)
- Dali Traditional Chinese Medicine Hospital (CN)
- Yunnan Provincial Department of Education (CN)
- Yunnan University of Traditional Chinese Medicine (CN)
- Chengdu University of Traditional Chinese Medicine (CN)
Publication Details
- Journal
- Metabolites
- Published
- 2025-02-03
- DOI
- https://doi.org/10.3390/metabo15020092
- Citations
- 3
- Primary Topic
- Berberine and alkaloids research
- Type
- article
- Field-Weighted Citation Impact
- 4.18
Funders
- National Natural Science Foundation of China
- China Academy of Chinese Medical Sciences
- Applied Basic Research Foundation of Yunnan Province
- National Key Research and Development Program of China