Biological properties study of bioactive diopside prepared from local raw materials

In this study, it was synthesized of ultrafine-structured diopside granules using the sintering method from solid raw materials (Dolomite) at a temperature of 1300 °C for 2 hours. Following the preparation, the pawders were homogenized within the [200-400 um] range and then immersed in simulated body fluid (SBF) at 37°C for 2, 7, 14 and 21 days. Following different soaking durations, the samples were carefully extracted from the fluids using deionized water. Subsequently, they were air-dried at room temperature prior to examine the impact of immersion on their crystalline properties. This involved monitoring the variation in ion concentration and pH during the immersion periods, as well as using X-ray diffraction (XRD), characterization through scanning electron microscopy (SEM), Fourier transform infrared (FTIR), and Inductively Coupled Plasma-Optical Emission Spectroscopy (ICP-OES) analysis. The findings revealed the partial dissolution of ultrafine Structured diopside granules and the development of a layer of carbonate-hydroxyapatite (Ca10.00P6.00O26.14H2.60C0.02) on the surface of the samples after seven days of immersion, with a granular size estimated at Dnm=102.96 nm. This volume continued to increase with longer dipping durations, reaching Dnm=205.94 nm for samples immersed for 21 days. Finally, the results obtained suggest that ultrafine-structured diopside granules are promising candidates for bone regeneration.