Effect of Flexible Plasma Pad on HaCaT Cells and Bacteria

ABSTRACT Dielectric barrier discharge (DBD) is a method that promotes wound healing. This study designed a flexible plasma pad with a polyimide film as the dielectric layer and patterned copper foil as the ground electrode. The flexible plasma pad was used to treat normal skin cells and bacteria at the wound site. Cell viability, intracellular and extracellular active substances, and Western blot analysis indicated that LTP can activate the PI3K/AKT/mTOR signaling pathway, decrease E‐Cadherin expression, and promote HaCaT cell proliferation. It exhibits antibacterial activity against Staphylococcus aureus and Escherichia coli . The results of this study suggest that this plasma pad is an efficient device for treating wounds and bacterial infections.