Dynamic Spread of Antibiotic Resistance Determinants by Conjugation to a Human-Derived Gut Microbiota in a Transplanted Mouse Model
Results show this ARG-bearing conjugative RP4 plasmid spread to diverse human gut bacterial taxa within a live animal where they persisted. These fluorescent marker strategies and human-derived microbiota transplanted mice provided a tractable model for investigating the dynamic spread of ARGs within gut microbiota and could be applied rigorously to varied microbiotas to understand conditions facilitating their spread.
Authors
- Linda S. Mansfield (ORCID: https://orcid.org/0000-0002-7523-7577)
- Daniel Vocelle (ORCID: https://orcid.org/0000-0003-0252-2673)
- A. Peer (ORCID: https://orcid.org/0009-0000-3661-2496)
- Azam A. Sher (ORCID: https://orcid.org/0000-0002-2607-1291)
- Charles E. Whitehead-Tillery
- Lixin Zhang (ORCID: https://orcid.org/0000-0002-1580-6723)
- Julia A. Bell (ORCID: https://orcid.org/0009-0007-7773-7114)
- Joshua T. Dippel
Institutions
- Michigan Department of Agriculture and Rural Development (US)
- Michigan State University (US)
Publication Details
- Journal
- Antibiotics
- Published
- 2025-02-04
- DOI
- https://doi.org/10.3390/antibiotics14020152
- Citations
- 4
- Primary Topic
- Clostridium difficile and Clostridium perfringens research
- Type
- article
- Field-Weighted Citation Impact
- 5.58