Acetic acid enabled nuclear contrast enhancement in epi-mode quantitative phase imaging
We show that the acetowhitening effect of acetic acid induces changes in refractive index composition that significantly enhance nuclear contrast in QPI. The application of qOBM with AA, along with the use of a Cycle-GAN algorithm to virtually stain tissues, highlights the potential of this approach for advancing label-free and slide-free, <i>ex vivo</i>, and <i>in vivo</i> histology.
Authors
- Amunet Jacobs
- Zhe Guang (ORCID: https://orcid.org/0000-0002-0500-8437)
- Paloma Casteleiro Costa (ORCID: https://orcid.org/0000-0002-7824-9805)
- Francisco E. Robles (ORCID: https://orcid.org/0000-0003-4413-9779)
- Zhenmin Li (ORCID: https://orcid.org/0009-0009-3670-664X)
Institutions
- Georgia Institute of Technology (US)
- University of Kentucky (US)
- The Wallace H. Coulter Department of Biomedical Engineering (US)
Publication Details
- Journal
- Journal of Biomedical Optics
- Published
- 2025-02-04
- DOI
- https://doi.org/10.1117/1.jbo.30.2.026501
- Citations
- 1
- Primary Topic
- Advanced X-ray Imaging Techniques
- Type
- article
- Field-Weighted Citation Impact
- 1.88
Funders
- National Science Foundation
- National Institutes of Health