Protocol for measuring the auxin-induced changes of m6A modification
Plant m<sup>6</sup>A modification is highly dynamic, with plant hormones serving as key regulators that induce these dynamics. Here, we present a protocol for detecting phytohormone-induced changes in m<sup>6</sup>A modification using RNA dot blot and m<sup>6</sup>A sequencing (m<sup>6</sup>A-seq) techniques. We describe steps for the auxin treatment, RNA dot blot and m<sup>6</sup>A-seq library preparation, and data analysis. This protocol holds potential applications for analyzing changes in m<sup>6</sup>A modification induced by plant hormones. For complete details on the use and execution of this protocol, please refer to Bin et al.<sup>1</sup>.
Authors
- Li Li
- Linjun Cai (ORCID: https://orcid.org/0000-0002-1095-1868)
- Chongsheng He (ORCID: https://orcid.org/0000-0003-1719-6794)
- Bin Li (ORCID: https://orcid.org/0000-0002-5717-1551)
- Lei Li (ORCID: https://orcid.org/0000-0001-8304-1286)
- Lan Li (ORCID: https://orcid.org/0000-0002-0151-8034)
- Yixing Li (ORCID: https://orcid.org/0009-0004-8918-7859)
Institutions
- Hunan University (CN)
- Hunan Normal University (CN)
- Hunan Academy of Agricultural Sciences (CN)
- China National Hybrid Rice R&D Central Hunan Hybrid Rice Reserch Center (CN)
- Hunan Rice Research Institute (CN)
- Xiangnan University (CN)
Publication Details
- Journal
- STAR Protocols
- Published
- 2025-02-04
- DOI
- https://doi.org/10.1016/j.xpro.2025.103629
- Primary Topic
- RNA modifications and cancer
- Type
- article
- Field-Weighted Citation Impact
- 0.00
Funders
- National Natural Science Foundation of China
- Natural Science Foundation of Hunan Province