Whole-genome amplification as a tool to improve bacterial detection by PCR in microbiological samples after endodontic treatment
The combination of WGA followed by PCR increased the detection of microorganisms from root canal samples after endodontic treatment using NaOCl as a CMP irrigant. So this combination of techniques can represent an important tool to improve the detection of microorganisms in endodontic research.
Authors
- Gabriella Augusto Pereira (ORCID: https://orcid.org/0009-0002-1558-8196)
- Leonardo Santos Antunes (ORCID: https://orcid.org/0000-0002-2115-6958)
- Helvécio Cardoso Corrêa Póvoa (ORCID: https://orcid.org/0000-0002-2759-2216)
- Gabriela Ceccon Chianca (ORCID: https://orcid.org/0000-0002-9539-0632)
- Natália Lopes Pontes Iorio (ORCID: https://orcid.org/0000-0001-5339-534X)
- Caroline Corrêa Fendeler
- Lívia Azeredo Alves Antunes
- Sérgio Pinto Chaves
Institutions
- Universidade Federal Fluminense (BR)
Publication Details
- Journal
- Frontiers in Oral Health
- Published
- 2025-02-03
- DOI
- https://doi.org/10.3389/froh.2025.1520945
- Citations
- 1
- Primary Topic
- Endodontics and Root Canal Treatments
- Type
- article
- Field-Weighted Citation Impact
- 1.79
Funders
- Coordenação de Aperfeiçoamento de Pessoal de Nível Superior
- Fundação Carlos Chagas Filho de Amparo à Pesquisa do Estado do Rio de Janeiro
- Universidade Federal Fluminense