ANTIBACTERIAL EFFECTIVENESS OF BIODEGRADABLE MEMBRANES CONTAINING TIGECYCLINE IN AN IN VIVO EXPERIMENT

Highlights Abdominal surgeries are complicated by an infectious process, the development of which significantly aggravates the underlying disease, lengthens the patient’s stay in the hospital, increases the cost of treatment, causes deaths and negatively affects the recovery time of operated patients. In order to prevent the formation of adhesions and the development of infection, it is promising to use anti-adhesion membranes that have their own antibacterial activity. Aim. To study the antibacterial activity of biodegradable membranes containing tigecycline upon implantation into an infected wound in a laboratory animal model. Methods. We used the following composition of biodegradable polymers for the manufacture of membranes: copolymer polylactide-co-glycolide (50:50) Mm 20-30 KDa and polylactide-co-glycolide (85:15). We dissolved the polymers were in 1,1,1,3,3,3-Hexafluoroisopropanol, and to impart antibacterial properties to the membrane, we added the antibiotic tigecycline to the membrane composition, and assessed the optimal concentration of the antibiotic. We prepared the membranes using electrospinning. To evaluate the antibacterial effectiveness of membranes when implanted into a wound infected with Staphylococcus aureus we used an animal model (rat). Results . The prepared membranes containing tigecycline at a concentration of 0.5 mg/mL solution exerted maximum antibacterial effect. When implanted into an infected wound in laboratory animals, membranes containing tigecycline effectively suppressed the infectious process. By the 14 th day of follow-up, we noted a complete healing of the wound, the absence of an inflammatory reaction, moreover we histologically confirmed an active infectious process. Conclusion . Biodegradable polymer membranes containing tigecycline as an antibacterial component effectively suppress the infectious process in an animal experiment.