Polysaccharides from <i>Annona Muricata</i> Leaves Protect Against H <sub>2</sub> O <sub>2</sub> -Induced Oxidative Stress in H9c2 Myoblasts
The purpose of this study was to investigate the antioxidant effects of <i>Annona muricata</i> leaf polysaccharide extract (ALPE) against oxidative stress induced by hydrogen peroxide (H<sub>2</sub>O<sub>2</sub>) in H9c2 myoblasts. The cells showed a cell viability of around 70% following exposure to 150 μM H<sub>2</sub>O<sub>2</sub>. In addition, ALPE was noncytotoxic to H9c2 myoblasts at 10-1000 μg/mL concentrations. As confirmed by MTT analysis and Annexin V/propidium iodide staining, ALPE treatment was found to protect H9c2 myoblasts exposed to H<sub>2</sub>O<sub>2</sub>. This protection is achieved by inhibiting reactive oxygen species levels and inducing superoxide dismutase and catalase activity. Furthermore, ALPE downregulated the activation of Bax, caspase-3, -8, and -9 but upregulated Bcl-2, thereby preventing H<sub>2</sub>O<sub>2</sub>-stimulated cytotoxicity in H9c2 myoblasts. ALPE activated the Nrf2/heme oxygenase-1 signaling cascade. Collectively, ALPE treatment decreased H<sub>2</sub>O<sub>2</sub>-induced oxidative stress. Therefore, ALPE can potentially be used as a natural resource with antioxidant properties.
Authors
- Bo-Gyeong Yoo (ORCID: https://orcid.org/0000-0002-8167-604X)
- Eui‐Hong Byun (ORCID: https://orcid.org/0000-0003-0220-7630)
- Jun-Pyo Hong (ORCID: https://orcid.org/0000-0002-4303-3107)
- Eui‐Baek Byun (ORCID: https://orcid.org/0000-0002-8612-6602)
- Bo Sun Kang
Institutions
- Korea Atomic Energy Research Institute (KR)
- Konyang University (KR)
- Kongju National University (KR)
Publication Details
- Journal
- Journal of Medicinal Food
- Published
- 2025-02-01
- DOI
- https://doi.org/10.1089/jmf.2024.k.0237
- Citations
- 2
- Primary Topic
- Traditional and Medicinal Uses of Annonaceae
- Type
- article
- Field-Weighted Citation Impact
- 2.22