Determination of total contents of flavonoids and phenolic fractions Pandanus julianettii Martelli

Pandanus julianettii Martelli is a type of plant endemic to mountainous areas in Papua that has long been used by people as a food ingredient. P. julianetti contains nutrients such as vitamin E, vitamin C, crude fiber, protein, carotene and fat; however, the research about the fruit is limited. The aim of this research is to carry out phytochemical screening tests and determine the total levels of flavonoids and phenolics of this pandanous in fruit. The method began with the extraction simplicia using ethanol, followed by fractionation using three variations of solvent: n-hexane, ethyl acetate and ethanol. The series of methods continued with phytochemical screening, then continued with determining the total flavonoid and phenolic percentage from the ethanol extract, ethyl acetate fraction and ethanol fraction. Determining the flavonoid content using the Chang method with quercetin as a comparison with standard. The results showed that the percentage of flavonoid in the extract was 0.58%, the ethyl acetate fraction was 0.44% and the ethanol fraction was 3.93%, while for the total content of phenolic of ethanol extract at 1.56%, the fraction of ethyl acetate 1.82% and ethanol fraction 1.20% calculated for gallic acid. This study found substantial levels of flavonoids and phenolics in extracts and solvent fractions derived from the fruit of the endemic Papuan plant P. julianetti. The enriched ethanol extract and fractions represent promising sources for further analysis of the nutritional and medicinal bioactives in this understudied of this fruit.