Development of solvent-cast antimicrobial PHBV films for the inhibition of spoilage microflora

Antimicrobial packaging based on poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV) incorporating Ethyl Lauroyl Arginate (LAE®) was developed and demonstrated effectiveness in inhibiting spoilage bacteria commonly encountered during household food storage. An exploratory study first evaluated the antimicrobial efficacy of nisin and LAE® against spoilage microflora from household environments. Due to its effectiveness against spoilage mixed cultures and resistance to organic solvents, LAE® was selected for further assessment and incorporation into active films. The minimum bactericidal concentration (MBC) of LAE® was determined against Listeria monocytogenes ATCC 19115 and Pseudomonas sp. isolated from spoiled almond beverage. Active PHBV-LAE® films were assessed for antimicrobial efficacy in both broth medium and almond beverage, showing substantial microbial inhibition. This study supports the potential of biodegradable active packaging with LAE® to reduce food waste throughout the food supply chain, both before and after package opening. • LAE and nisin were tested against environmental spoilage bacteria mixes. • LAE was the most effective for release after opening. • PHBV-LAE films ensured total microbial inhibition in broth medium. • PHBV-LAE films caused 2.6-log reduction of Pseudomonas sp. in almond beverage. • Active agent release during storage after opening help prevent domestic food waste.