Comparative Study of Antibacterial activity of neem leaf extract against standard benzyl penicillin by using In vitro cup plate method

This research article is based on the antibacterial activity of neem. We compared the antibacterial activity of a standard antibiotic with our neem alcoholic extract using the cup-plate method. For this study, we utilized non-pathogenic bacteria, specifically Lactobacillus grown in MRS agar media prepared with tomato juice and meat extract. This bacterial culture was employed to evaluate the antibacterial activity of neem leaf extract. The entire study was conducted in a college laboratory to understand the concepts of microbial assay and the format of research paper writing. The benefit of using neem extract lies in its easy availability and low cost. However, neem is not used commercially due to its bitter taste and the challenging extraction process.