In vitro anti-cancer activity of siddha metallo mineral formulation from Thanga uram with Hela cell lines

The second most common malignant tumour in women worldwide, cervical cancer seriously jeopardizes their health. It has been established that a high-risk Human papillomavirus (HPV) infection is the cause of cervical cancer. Cervical cancer is associated with several risk factors, including reproductive, behavioural and sexual characteristics. Many Siddha practitioners often prescribe Thanga uram (TU), a Siddha metallo-mineral formulation, for genito-urinary diseases, infertility, varicose veins and leucorrhoea. This study aims to evaluate the anti-cancer activity of "Thanga uram" against HeLa cell lines. Hela cell lines were used to assess the antiproliferative activity TU using the MTT assay and apoptosis by Annexin V flow cytometry. Thanga uram produced dose-dependent cytotoxic effects against HeLa cell lines with an LC50 value of 145.891µg/mL. Further investigation, using Annexin V flow cytometry, equivalently revealed the late apoptotic effect, with a percentage value of 28.15. Thus, the current study evaluates the effectiveness of Thanga Uram against cervical cancer.